Multiparameter Flow Cytometry
Flow cytometry is widely used for the diagnosis and monitoring of hematological disorders including multiple myeloma. We have developed different approaches of multiparameter flow cytometry to detect various malignant and normal plasma cells, B and T lymphocytes, NK cells, hematopoietic stem cells…
We can combine these approaches with BrDU incorporation or Ki67 staining to analyze tumoral or normal cell proliferation in various cell lines or in samples from patients with different hematological cancers.
Cells are analyzed using our cytometer (Beckman Coulter’s CytoFlex, 3 lasers, 12 colors).
Diag2Tec uses also a cell sorter (FACS Aria) to isolate and recover one or more cell population(s) according to size, granularity or fluorescence parameters.
Analyses and Applications
To analyze the flow cytometry, we use FlowJo software or the CytoFlex’s software.
We use flow cytometry to study different pathways such as apoptosis, cell cycle, DNA damage in various cell lines and primary samples from patients.
We can also perform quantification of membrane proteins in different populations of cells.
Additionally, we can investigate the effect of your drug or antibody in tumoral and non-tumoral cells.
